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Advances in Dental Research
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Adv Dent Res 11:420-425, November, 1997
© 1997 SAGE Publications

Application of Transverse Microradiography for Measurement of Mineral Loss by Acid Erosion

A.F. Hall

Glasgow Dental Hospital and School 378 Sauchiehall Street Glasgow G2 3JZ, Scotland, UK

J.P. Sadler

Glasgow Dental Hospital and School 378 Sauchiehall Street Glasgow G2 3JZ, Scotland, UK

R. Strang

Glasgow Dental Hospital and School 378 Sauchiehall Street Glasgow G2 3JZ, Scotland, UK

E. De Josselin De Jong

Inspektor Research Systems BV Quellijnstraat 92 1072 XX Amsterdam The Netherlands

R.H. Foye

Glasgow Dental Hospital and School 378 Sauchiehall Street Glasgow G2 3JZ, Scotland, UK

S.L. Creanor

Glasgow Dental Hospital and School 378 Sauchiehall Street Glasgow G2 3JZ, Scotland, UK

This paper describes a novel application of transverse microradiography for the detection and quantification of mineral loss due to acid erosion in thin tooth sections. Sixty-four specimens were randomly divided into eight equal-sized groups and exposed to an orthophosphoric-acid-based erosive fluid (pH = 3) for 0, 0.25, 0.5, 1, 2, 5, 12, or 24 hrs. We made microdensitometric scans separately across both enamel and dentin to derive data for the total mineral loss and the minimum mineral content within the eroded area. We then analyzed specimens using a profilometer to determine (1) the area above a plot of penetration depth against distance and (2) the maximum depth of penetration. Correlation coefficients for comparisons between microradiographic and profilometric data for both enamel and dentin specimens varied between 0.87 and 0.96. Two-sample t tests demonstrated that the microradiographic technique could detect early erosion, i.e., discriminate between erosion times of less than 1 hr. It was concluded that this application of transverse microradiography was a useful and acceptable method for the measurement of early mineral loss in vitro, occurring as a result of acid erosion.

Key Words: Erosion • transverse microradiography • profilometry • quantification.

Advances in Dental Research, Vol. 11, No. 4, 420-425 (1997)
DOI: 10.1177/08959374970110040701


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