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Adv Dent Res 11:100-109, April, 1997
© 1997 SAGE Publications
Analysis of Gene Expression in Streptococcus Mutans in Biofilms in Vitro
Robert A. Burne
Department of Dental Research University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue Rochester, New York 14642, Department of Microbiology and Immunology University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue Rochester, New York 14642
Yi-Ywan M. Chen
Department of Dental Research University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue Rochester, New York 14642
Jana E.C. Penders
Department of Dental Research University of Rochester School of Medicine and Dentistry 601 Elmwood Avenue Rochester, New York 14642
The purpose of this study was to develop methods for the consistent production of biofilms of S. mutans containing reporter gene fusions, and to examine the expression of genes involved in sucrose metabolism in adherent populations of this organism. Three strains of S. mutans harboring reporter gene fusions to the gene promoter regions of the gtfBC genes, ftf, and scrA were grown in a Rototorque biofilm fermenter in a tryptone-yeast extract-sucrose medium. Quasi-steady-state levels of reporter gene activity were measured after the biofilms were grown for either 48 hrs or 7 days. Also, induction of gene expression by the addition of sucrose to biofilm cells was monitored. Reporter gene activity was measurable from all gene fusion strains. This study (i) establishes the feasibility of doing detailed molecular and physiologic studies on immobilized populations of S. mutans, (ii) demonstrates that the polysaccharide synthesis machinery of S. mutans is differentially expressed in biofilms, and (iii) opens the way for a more detailed analysis of the environmental signals and signal transduction pathways governing the regulation of gene expression by S. mutans cells that are immobilized on a solid surface.
Key Words: Glucan • fructan • dental caries • gene fusion • gene regulation.
Advances in Dental Research, Vol. 11, No. 1,
100-109 (1997)
DOI: 10.1177/08959374970110010101
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